ABSTRACT
Abstract Infection by the acantocephalan Neoechinorhynchus buttnerae is considered one of most important concerns for tambaqui fish (Colossoma macropomum ) production. Treatment strategies have been the focus of several in vivo studies; however, few studies have been undertaken on in vitro protocols for parasite maintenance. The aim of the present study was to develop the best in vitro culture condition for N. buttnerae to ensure its survival and adaptation out of the host to allow for the testing of substances to be used to control the parasite. To achieve this, parasites were collected from naturally infected fish and distributed in 6-well culture plates under the following treatments in triplicate: 0.9% NaCl, sterile tank water, L-15 Leibovitz culture medium, L-15 Leibovitz + agar 2% culture medium, RPMI 1640 culture medium, and RPMI 1640 + agar 2% culture medium. The plates containing the parasites were maintained at 24 °C, 28 °C, and 32 °C. The RPMI 1640 + agar 2% culture medium showed the best survival of 24 days at 24 °C. No body alterations such as swollen parasites, body deformation, dehydration and hardening were observed in the RPMI 1640 + 2% culture medium.
Resumo A infecção pelo acantocéfalo Neoechinorhynchus buttnerae é considerada uma das preocupações mais importantes para produção de tambaqui (Colossoma macropomum). Estratégias de tratamento têm sido o foco de vários estudos in vivo ; entretanto, poucos estudos foram realizados em protocolos in vitro para manutenção do parasito. O objetivo deste estudo foi desenvolver a melhor condição de cultura in vitro para N. buttnerae para garantir sua sobrevivência e adaptação fora do hospedeiro, a ser utilizado para teste com substâncias no controle do parasito. Para isso, os parasitos foram coletados de peixes naturalmente infectados e distribuídos em placas de cultura de 6 poços sob os seguintes tratamentos em triplicata: 0.9% NaCl, água estéril do tanque, meio de cultura L-15 Leibovitz, meio de cultura L-15 Leibovitz + ágar 2%, meio de cultura RPMI 1640, e meio de cultura RPMI 1640 + ágar 2%. As placas contendo os parasitos foram mantidos a 24 °C, 28 °C, e 32 °C. O meio de cultura RPMI 1640 + ágar 2% apresentou a melhor sobrevivência de 24 dias a 24 °C. Nenhuma alteração corporal tais como inchaço dos parasitos, deformação corporal, desidratação e endurecimento foram observados no meio de cultura RPMI 1640 + ágar 2%.
Subject(s)
Animals , Temperature , Acanthocephala/growth & development , Culture Media , Characiformes/parasitologyABSTRACT
Adult worms of Rhadinorhynchus dorsoventrospinosus [Acanthocephala: Rhadinor-hynchidae] were collected from the small intestine of the red spot emperor Lethrinus lentjan [family Lethrinidae] from locations along the Red Sea at Jeddah City, Saudi Arabia. Twenty three out of 70 fish specimens [32.9%] were found to be naturally infected. The parasite was described using photo research Zeiss microscopy and a scanning electron microscope [SEM]. Light microscopic studies revealed that the adult worm possessed a proboscis which was long, cylindrical with a uniform width measured 0.44 +/- 0.02 [0.38-0.46] mm in length and 0.12 +/- 0.02 [0.09-0.15] mm in width. Proboscis hooks observed by scanning electron microscopy were large, uniform in size [14-16 rows of 26 hooks each] with a row of longer hooks at the base. Comparison between the present described species and four species of the same genus was done, it was observed that there was only one comparable species, R. dorsoventrospinosus resembled the present parasite in the general morphology and differed from others, so the present studied species is classified as R. dorsoventrospinosus with new host and locality records
Subject(s)
Acanthocephala/growth & development , Acanthocephala/anatomy & histology , Microscopy, Electron, ScanningABSTRACT
En el presente trabajo, se da a conocer el resultado de la infección experimental del perro doméstico Canis familiaris, con Corynosoma obtuscens Lincicome, 1943, un acantocéfalo de gran capacidad infectiva y de inespecificidad para el hospedero definitivo, que lo convierte en un parásito potencial para el hombre, quien consume pescado marino semicrudo bajo la forma de "cebiche", plato típico de nuestro país. Encontrándose por primera vez la presencia de huevos maduros de C. obtuscens en las heces de un hospedero no habitual.